Neat1 expression in subpopulations of white adipocytes and adipocyte precursors in iWAT. (A) UMAP visualization of adult iWAT cell types and associated Neat1 expression from reanalyzed public data sets (GSE133486). Colors denote distinct cell types, including white adipocytes (Adipo), adipocyte precursors (APC1–4), B cells (Bcell1–3), T cells (Cd4 and Cd8), macrophages (Macro1–3), and endothelial cells (Endo), illustrating that Neat1 expression is prevalent among subpopulations of white adipocytes and adipocyte precursor cells. (B) Simultaneous detection of Neat1 and pre-mRNAs of cell type–specific markers using intron-targeted probes. White adipocytes and adipocyte precursor cells were identified by the expression of Pparg and Pi16, respectively. The panels indicate the percentages of these cells expressing Neat1. Scale bar, 5 µm. (C) Proportions of adipocyte precursors, endothelial cells, and M1/M2 macrophages in iWAT of WT and Neat1 KO mice, with no statistically significant differences due to KO of Neat1 observed (Mann–Whitney U-test, n = 6 per group).
