FIGURE 4.
Formation of enlarged paraspeckles in iWAT upon cold exposure. (A) Subnuclear localization of Neat1_1 and Neat1_2 in iWAT. Localization of Neat1_1 becomes restricted to paraspeckles where Neat1_2 accumulates upon cold exposure. (B) Quantification of the number of foci per cell and the area of each focus positive for Neat1_1/2 or Neat1_2. The asterisks indicate statistically significant differences: (*) P < 0.05, (***) P < 0.001, Mann–Whitney U-test. (C) Simultaneous detection of Neat1_2 and the paraspeckle marker protein Nono in adipocytes of iWAT upon cold exposure. Scale bar, 5 µm. Dotted lines represent the shape of each nucleus.
