Transient upregulation of Neat1_2 during early stages of beige adipocyte differentiation upon cold exposure. (A) Schematic representation of the positions of the probes that detect both Neat1_1 and Neat1_2 (Neat1_1/2) and solely Neat1_2. (B) Northern blot analyses of Neat1_1 and Neat1_2 expression in iWAT and BAT at 0 h, 4 h, 24 h, and 4 days after cold exposure. rRNA was used as a loading control, visualized using ethidium bromide–stained agarose gels. The asterisk indicates nonspecific signals detected by the Neat1_1/2 probe. (C) qRT-PCR analyses of Neat1_1/2, Neat1_2, and Ucp1 expression in iWATs at various time points post–cold exposure. Note the earlier upregulation of Neat1_2 compared to that of Ucp1. The asterisks indicate statistically significant changes: (*) P < 0.05, bootstrap test compared with “0 h” followed by post hoc Bonferroni correction, 1000 surrogates, n = 3 per group. (D) Western blot analyses of tyrosine hydroxylase (TH) expression in sympathetic ganglia of WT and Neat1 KO (KO) mice. Tardbp was used as a loading control.
