Brown adipocyte differentiation appears fairly normal in Neat1 KO mice. (A) Nomarski images of H&E-stained paraffin sections of BAT derived from WT and Neat1 KO mice reared under normal ambient temperature (RT) and cold temperature (cold) for 1 week. No apparent differences were observed between WT and Neat1 KO BAT. (B) Immunohistochemical detection of Ucp1 in BAT derived from WT and Neat1 KO mice reared under normal (RT) and cold temperature (cold) for 1 week. Note the presence of Ucp1 expression in both conditions. (C) Western blot analyses of Ucp1 expression in BAT of WT and Neat1 KO animals. Two biological replicates (rep1 and rep2) were used for each condition. CBB-stained gels served as a loading control. Scale bar, 20 µm. (D) Expression of mRNAs for beige cell markers Ucp1, Elovl3, Cidea, Cox4, Pgc1a, and Eva1. The asterisks indicate statistically significant changes: (*) P < 0.05, bootstrap test, 1000 surrogates, n = 2 for RT and n = 3 for cold.
