Mapping RNA–protein interactions with subcellular resolution using colocalization CLIP

HuR exhibits unique binding preferences within stress granules. (A,B) Venn diagrams depict the overlap of peaks for all coCLIP data sets from either mock (A) or arsenite (B) stress conditions. (C,D) Scatterplots contrasting enriched coCLIP to input CLIP for mRNA-binding events as log₂ fold changes, comparing G3BP1 coCLIP versus nuclear NLS coCLIP under mock (C) and arsenite (D) stress conditions. Side histograms depict the relative density of each feature and are scaled for comparison within each feature and across mock and arsenite stress. (E,F) Scatterplots as in C,D comparing G3BP1 coCLIP versus cytoplasmic NES coCLIP under mock (E) and arsenite (F) stress conditions.