A new reagent for in vivo structure probing of RNA G and U residues that improves RNA structure prediction alone and combined with DMS

Example 16S and 23S rRNA stems from Escherichia coli comparing ETC and DMS prediction accuracy. DMS reactivity at As and Cs and ETC reactivity at Gs and Us were mapped onto the known structure of rRNA. Reactivity values are provided in the legend. (A) 16S central domain stems 25 and 26, (B) 23S domain V stems 76–78, (C) 23S domain III stems 57 and 58, and (D) 16S 5′ domain stems 3, 4, 15, and 16. Supplemental Figures S9–S12 provide accompanying circle diagrams for sequence alone, individual chemicals, and combined chemicals.