A new reagent for in vivo structure probing of RNA G and U residues that improves RNA structure prediction alone and combined with DMS

Comparison of RT enzymes for sequencing libraries of Escherichia coli 16S rRNA and 23S rRNA probed in vivo with ETC. (A,B) Violin plots showing background-subtracted mutation rates for paired and unpaired (A) Us and (B) Gs. Medians are provided in Supplemental Table S2. The significance of Wilcox tests is denoted with asterisks comparing paired and unpaired residues, and P-values are provided in Supplemental Table S3. The upper and lower portions of some violins were clipped to better visualize the data. Temperatures are for the extension step of RT. (C,D) ROC curves of background-subtracted mutation rates for (C) U and (D) G for each enzyme.