FIGURE 9.
Application of ROCKET in the preparation of catalytically active CPEB3 ribozyme. (A) Oligonucleotide sequences selected by ROCKET with the portions covering T7 promoter (T7 pro, gray), leader (dark blue), and CPEB3 ribozyme (light blue) highlighted. (B) Secondary structure of the produced transcript. The cleavage site of the CPEB3 ribozyme (between A-1 and G1) is indicated by an arrow. (C) Synthesis and self-cleavage of the CPEB3 ribozyme was monitored by 10% denaturing PAGE as described in the Materials and Methods.
