FIGURE 7.
In vitro transcription of all T. kodakarensis tRNA genes. DNA templates were based on tRNA genes retrieved from the genomic tRNA database (Chan and Lowe 2016) and constructed with oligonucleotides selected as optimal by the ROCKET software (Supplemental Table S1). RNAs were visualized by Toluidine blue staining after 10% denaturing PAGE of 5 µL transcription mixture. Nucleotides at positions 1 and 72 in those tRNAs that do not have a G residue as the 5′ end (marked with an asterisk), have been substituted with G1-C72.
