Rational design of oligonucleotides for enhanced in vitro transcription of small RNA

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FIGURE 6.
FIGURE 6.

In vitro RNase P reaction on precursor tRNA. (A) A cloverleaf structure of precursor tRNA. (B–D) Cleavage of the leader sequence by RNase P. Based on optimal, ROCKET-selected oligonucleotides precursors for (B) tRNAGln-CTG, (C) tRNAGln-TTG, and (D) tRNAiMet-CAT were in vitro synthesized and subsequently incubated with (+) or without RNase P (−). The RNAs were visualized by Toluidine blue staining after 10% denaturing PAGE (7 M urea). The asterisk marks tRNAs used as a migration control.

This Article

  1. Published in Advance February 29, 2024, doi: 10.1261/rna.079923.123 RNA 30: 710-727

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