Rational design of oligonucleotides for enhanced in vitro transcription of small RNA

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FIGURE 5.
FIGURE 5.

Characterization of the transcription reaction. (A) RNA yields obtained by in vitro transcription at specific time points were analyzed by Toluidine blue staining after 10% denaturing PAGE and (B) relative band intensities were quantified as described in the Materials and Methods and fitted to a single exponential curve (Equation 4). Errors, SD (n = 3) are shown.

This Article

  1. Published in Advance February 29, 2024, doi: 10.1261/rna.079923.123 RNA 30: 710-727

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