Application of ROCKET to the synthesis of EGFP protein. (A) Computational workflow for design of DNA oligonucleotides for the EGFP gene. (B) Experimental workflow for EGFP synthesis. The experiment was perfomed by two successive PCRs. The resulting template DNA was gel-purified and then used for the protein synthesis. (C) One microliter of the PCR product from each PCR step was analyzed by 2% agarose gel electrophoresis. The gel was stained by SYBR gold. (D) EGFP synthesis was monitored by fluorescence intensities where EGFP was excited by the 488 nm light, and the emission at 510 nm was measured. Errors (x) from three biological replicates, SD, and P-values are shown.
