High-throughput quantitation of protein–RNA UV-crosslinking efficiencies as a predictive tool for high-confidence identification of RNA-binding proteins

Canonical RBPs display high UV-crosslinking efficiencies. (A) Violin plots (equal area) with box plot and 95% confidence diamond overlays showing estimated UV-crosslinking efficiencies (purple) of GO-annotated RBPs (GO:0003723), proteins lacking GO-annotated RNA-binding, and their observed abundances (log₁₀[%TP]) in input (blue) and clRNP (red) fractions. Corresponding pie charts show the proportion of proteins within each %CL bin. (B) Comparison (A) of ribosomal proteins (PC00202), chaperones (PC00072), and canonical RBPs containing at least one of the indicated RNA-binding domains (Pfam, see Source Data). (C) Comparison (A) of ribosomal proteins (B) containing additional GO-annotation as cytosol (GO:0005829) or mitochondrion (GO:0005739) cellular component. The resulting ribosomal protein lists were curated to correct for missing or false GO-annotation.