High-throughput quantitation of protein–RNA UV-crosslinking efficiencies as a predictive tool for high-confidence identification of RNA-binding proteins

Top 15 and bottom 15%CL ranked Pfam families containing three or more candidate RBPs with estimated UV-crosslinking efficiencies. (A) Violin plots (equal area) with box plot and 95% confidence diamond overlays showing estimated UV-crosslinking efficiencies (purple) of proteins within each of the indicated Pfam families and their observed abundances (log₁₀[%TP]) in input (blue) and clRNP (red) fractions. Corresponding pie charts show the proportion of proteins within each %CL bin.