New directions for Ψ and m1A decoding in mRNA: deciphering the stoichiometry and function
- Meiling Zhang1,5,
- Xiaoting Zhang1,5,
- Yichen Ma2,3 and
- Chengqi Yi1,3,4
- 1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
- 2Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
- 3Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
- 4Department of Chemical Biology and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Corresponding author: chengqi.yi{at}pku.edu.cn
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↵5 These authors contributed equally to this work.
Abstract
Over the past decade, advancements in epitranscriptomics have significantly enhanced our understanding of mRNA metabolism and its role in human development and diseases. This period has witnessed breakthroughs in sequencing technologies and the identification of key proteins involved in RNA modification processes. Alongside the well-studied m6A, Ψ and m1A have emerged as key epitranscriptomic markers. Initially identified through transcriptome-wide profiling, these modifications are now recognized for their broad impact on RNA metabolism and gene expression. In this Perspective, we focus on the detections and functions of Ψ and m1A modifications in mRNA and discuss previous discrepancies and future challenges. We summarize recent advances and highlight the latest sequencing technologies for stoichiometric detection and their mechanistic investigations for functional unveiling in mRNA as the new research directions.
Keywords
This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
