New directions for Ψ and m1A decoding in mRNA: deciphering the stoichiometry and function
- 1 Peking University School of Life Sciences;
- 2 Peking University Peking-Tsinghua Center for Life Sciences
- ↵* Corresponding author; email: chengqi.yi{at}pku.edu.cn
Abstract
Over the past decade, advancements in epitranscriptomics have significantly enhanced our understanding of mRNA metabolism and its role in human development and diseases. This period has witnessed breakthroughs in sequencing technologies, and the identification of key proteins involved in RNA modification processes. Besides the extensively studied m6A, Ψ and m1A modifications have risen as important epitranscriptomic marks. Alongside the well-studied m6A, Ψ and m1A have emerged as key epitranscriptomic markers. Initially identified through transcriptome-wide profiling, these modifications are now recognized for their broad impact on RNA metabolism and gene expression. In this Perspective, we focus on the detections and functions of Ψ and m1A modifications in mRNA and discuss previous discrepancies and future challenges. We summarize recent advances and highlight latest sequencing technologies for stoichiometric detection and their mechanistic investigations for functional unveiling in mRNA as the new research directions.
- Received January 15, 2024.
- Accepted February 9, 2024.
- Published by Cold Spring Harbor Laboratory Press for the RNA Society
This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
