Fungi of the order Mucorales express a “sealing-only” tRNA ligase

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FIGURE 2.
FIGURE 2.

The predicted structures of Mucorales Trl1 resemble the ligase domain of fungal tRNA ligase. (A) X-ray crystal structure of the ligase domain of the ascomycete fungus C. thermophilum (6N0T). (B) The AlphaFold predicted the structure of R. arrhizus sealing-only Trl1. (C) The AlphaFold predicted the structure of M. circinelloides sealing-only Trl1. (D) Superimposition of X-ray crystal and AlphaFold predicted structures from A to C. Colors are as in A–C. For R. arrhizus RMSD is 0.913 Å over 247 Cα atoms; for M. circinelloides it is 0.901 Å over 244 Cα atoms. (E) Superimposition of X-ray crystal and Phyre predicted structures. Colors are as in A–C. (F) Superimposition of X-ray crystal structures of Trl1 ligase from C. thermophilum (6N0T) and T4 RNA ligase (2C5U; plum colored). The amino- and carboxy-terminal subdomains are indicated in each panel.

This Article

  1. Published in Advance February 2, 2024, doi: 10.1261/rna.079957.124 RNA 30: 354-366

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