FIGURE 5.
Analysis of snRNA nuclear localization in HeLa cells. (A) RT-qPCR analysis of canonical and variant U1 snRNAs in nuclear and cytoplasmic fractions from HeLa cells cotransfected with Dup51p reporter and either pcDNA or U1-5a constructs for canonical and variant U1 snRNA. The level of snRNAs in the total or nuclear fraction RNA was calculated relative to the cytoplasmic fraction (±SD; n = 3). (B) Agarose gel analysis of the RT-qPCR products from total, nuclear, and cytoplasmic RNA fractions. Sizes of fragments in a 100 bp DNA ladder are indicated. Sequences of primer sets used for RT-qPCR are provided in Supplemental Table S2.
