Examining the capacity of human U1 snRNA variants to facilitate pre-mRNA splicing

Context of the RNU1-1 gene regulatory elements improves the expression of vU1 snRNAs in HeLa cells. (A) Expression levels of vU1 snRNAs, calculated relative to the endogenous canonical U1 after normalization to the U2 snRNA (n = 3), are shown for HeLa cells transfected with either pcDNA or pNS6U1 plasmids harboring vU1 genes. (B) Agarose gel analysis of amplified RT-qPCR products for HeLa cells transfected with either pcDNA or pNS6U1 plasmids harboring vU1 genes. Sizes of fragments in a 100 bp DNA ladder are indicated. Sequences of primer sets used for RT-qPCR are provided in Supplemental Table S2.