Genome-wide kinetic profiling of pre-mRNA 3′ end cleavage

3′ end cleavage half-lives are associated with RNA polymerase dynamics at the 3′ end of the gene. (A) Cleavage half-lives (y-axis) versus the lengths of readthrough regions (in kb, x-axis), with a linear fit (blue). (B) Metagene plot of normalized RNA Pol lI ChIP-seq read coverage (y-axis) in a 1000 nt region centered on constitutively used CSs (x-axis), with sites separated by quartiles of cleavage half-lives (blue lines). (C) Metagene plot of normalized 4sU-seq read coverage for the 5m 4sU labeling time point (y-axis) in a 1000 nt region centered on constitutively used CSs (x-axis), with sites separated by quartiles of cleavage half-lives (blue lines).