Identification, characterization, and structure of a tRNA splicing enzyme RNA 5′-OH kinase from the pathogenic fungi Mucorales

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FIGURE 6.
FIGURE 6.

RNA kinase versus DNA kinase activity. Reaction mixtures (10 µL) containing 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MgCl2, 0.1 mM GTP, 100 nM (1 pmol) 10-mer 5′-OH RNA or DNA substrate 3′-labeled with Cy5 (depicted at top), and MciKIN as specified were incubated at 37˚C for 5 min. The reactions were quenched with formamide/EDTA, and the products were analyzed by urea-PAGE.

This Article

  1. Published in Advance October 2, 2024, doi: 10.1261/rna.080247.124 RNA 30: 1674-1685

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