The requirement for DXO/Dxo1/Rai1 for 25S′ processing is not conserved across kingdoms. Shown is next-generation sequence data for RNAs with a 5′ monophosphate mapped to the rDNA repeat. This analysis defines the 5′ end of the 18S small subunit RNA (ssRNA) (A, D, and G), 5.8S RNA (B, E, and H) and 25S/28S large subunit rRNA (lsRNA) (C, F, and I), which are unaffected by a null allele of the sole DXO/Dxo1/Rai1 family member in H. sapiens (A–C), A. thaliana (D–F), and S. pombe (G–I). In contrast, our previous published data (J) show a specific defect in 25S rRNA processing in S. cerevisiae (Hurtig and van Hoof 2022). This panel is in gray scale instead of color to distinguish it as previously published.
