A circular split nanoluciferase reporter for validating and screening putative internal ribosomal entry site elements

Circular split nLuc reporter can be used to test putative endogenous circular RNA IRESs. (A) Luminescence from HEK293T cells transfected with the circular split nLuc reporter with a putative circular RNA cellular IRES. (B) Luminescence from HEK293T cells transfected with the circular split nLuc reporter with the circZNF609 IRES, the EMCV IRES, or a 42 nt random sequence negative control. (RLU) Relative luminescence units. Data are presented as mean values +/− one SD (n = 3 biological replicates). Significance was calculated using unpaired two-tailed Student's t-test. (****) P < 0.0001, (***) P < 0.001, (**) P < 0.01, (*) P < 0.05, (n.s.) P > 0.05.