A conditional loss-of-function allele for Vaultrc5. (A) Schematic representation of conditional Vaultrc5 locus (Vaultrc5flx) and its recombination after Cre expression to generate a null allele. The position of the three primers used for monitoring the recombination is highlighted. When used in combination, these result in amplicons of 309 base pairs (bp) for wild-type alleles (P1/P2) and 331 bp for recombined alleles (P1/P3). An example gel is shown on the bottom, showing the recovery of heterozygous null animals (+/−) following β-actin-Cre expression. (B) Genotyping PCR showing the recovery of wild-type (+/+), heterozygous (+/−), and Vaultrc5-null (−/−) animals from Vaultrc5+/− intercrosses. (C) Relative levels of Vaultrc5 in mouse embryonic fibroblasts isolated from wild-type, heterozygous, and homozygous null animals. (D, top) Schematic representation of the Vaultrc5 locus and its distance to its conserved upstream and downstream neighbors. (Bottom) Expression of Vaultrc5 flanking genes in brains from wild-type and null animals. (E) Observed and Expected numbers for wild-type (+/+), heterozygous (+/−), and Vaultrc5-null (−/−) animals obtained from Vaultrc5+/− intercrosses. P-value was calculated with a χ2 test. (F) As in E but for animal sex.
