Structure and conservation of the murine Vaultrc5 locus. (A) Minimum free energy secondary structure prediction for Vaultrc5 and vtRNA1-1 transcripts. The position of the internal regulatory elements is highlighted. The sequences that are not conserved between the mouse and human transcripts are shown in gray in the vtRNA1-1 structure. (B) Position of known functional elements on vtRNA1-1, including the noncanonical miRNAs (blue, sequences shown on the bottom) and p62 interaction region (magenta). (C) Genome browser view of sequencing reads over Vaultrc5 and pre-mmu-miR-19a loci in multiple adult mouse tissues. (D) Expression levels of the predicted Vaultrc5-derived miRNAs compared to those of miR-19a (miR-19a-3p) and its passenger strand (mir-19a-5p) across two sequencing replicates.
