TABLE 1.
RNAs enriched and sequenced by MAPS using SprC as a bait
| MAPS experiment | In silico affinity predictions | ||||
|---|---|---|---|---|---|
| Genes | Functions | Fold change MS2-SprC/MS2 | P-value | Predicted energy ΔΔG (kcal/mol) |
|
| NWMN_2042 | deoD | Purine nucleoside phosphorylase | 35.5 | 3.8 × 10−30 | −9.7 |
| NWMN_0430 | – | Conserved hypothetical protein | 9.1 | 4.3 × 10−08 | −16.3 |
| NWMN_2050 | czrB | Cation efflux family protein | 6.4 | 1.7 × 10−04 | −13.5 |
| NWMN_0655 | mgrA | MarR family regulatory protein | 6.2 | 1.9 × 10−12 | −4.9 |
| NWMN_2333 | bcr/CflA | Drug resistance transporter | 4.8 | 2.4 × 10−03 | −11.8 |
| NWMN_1138 | fapR | Fatty acid and phospholipid biosynthesis transcriptional regulator | 4.75 | 0.016 | −9.3 |
| NWMN_1547 | rpmA | 50S ribosomal protein L27 | 4.2 | 6.4 × 10−08 | −5.5 |
| NWMN_1943 | agrB | Accessory gene regulator protein B | 4.1 | 6.4 × 10−08 | −7.4 |
| NWMN_0402 | – | Hypothetical protein | 3.6 | 1.3 × 10−05 | −8.1 |
| NWMN_1548 | – | Hypothetical protein | 3.4 | 0.012 | −12.5 |
| NWMN_0248 | bmQ2 | Branched-chain amino acid transport system II carrier protein | 3.3 | 0.012 | −10.4 |
| NWMN_1382 | huP | DNA-binding protein HU | 3.2 | 7.2 × 10−06 | −5.9 |
| NWMN_1945 | agrC | Accessory gene regulator protein C | 2.5 | 3.2 × 10−03 | −9.4 |
| NWMN_1134 | rpmB | 50S ribosomal protein L28 | 2.5 | 0.033 | −6.5 |
-
The data are the mean of three independent biological replicates. Cutoffs were set to a fold-change > 2 and a P-value <0.05. Predicted energy refers to the energy of the sRNA-mRNA target duplexes calculated by the IntaRNA software (Mann et al. 2017).
