RT-qPCR analysis of czrB relative expression as a function of SprC expression after phagocytosis. Relative transcript level of (A) SprC and (B) czrB during different internalization times, determined with gyrB as reference gene. Phagocytosis assays were performed in Newman strain for 2 h, 10 h, 18 h, and 24 h before lysis of macrophages and RNA extraction. A control (CTRL) was performed with Newman strain growth in macrophage medium for 2 h. Relative expression level in extracellular bacteria (ECB) was also determined after 2 h of bacteria-macrophages contact. (C) Relative transcript levels of czrB as a function of SprC expression at 2 h of internalization, determined with gyrB as reference gene. The data were reproduced by four independent biological replicates with Mann–Whitney test for statistical analysis ([*] P < 0.05, [**] P < 0.01).
