FIGURE 4.
Western blot analysis of czrB expression levels as a function of SprC expression. Relative quantification of CzrB levels determined by western blot and using SpA as an internal loading control. Bacteria were grown for 2 h before addition of 5 mM of ZnSO4, and protein extractions were performed after (A) 4 h and (B) 6 h of additional growth. Values are the mean of four (after 4 h) or three (after 6 h) independent experiments. Mann–Whitney test or Student t-test were used for statistical analysis ([*] P < 0.05, [**] P < 0.01, [***] P < 0.005).
