Northern blot analysis of czrAB transcript level and half-life determination. (A) Northern blot analysis of czrAB transcript level during bacterial growth as a function of SprC expression. (B) Relative quantification of czrAB transcript levels determined by northern blot and using tmRNA as an internal loading control. (C) Relative quantification of czrAB transcript levels during bacterial growth in the presence of rifampicin determined by northern blot and using tmRNA as an internal loading control. The data were reproduced by three independent biological replicates and analyzed with Student t-test (B, 2–6 h and then 8 h) or Mann–Whitney test (B, 1 h and 7 h) and paired samples t-test (C) for statistical analysis ([*] P < 0.05, [**] P < 0.01, [***] P < 0.005).
