The antivirulent Staphylococcal sRNA SprC regulates CzrB efflux pump to adapt its response to zinc toxicity

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FIGURE 2.
FIGURE 2.

Electrophoretic mobility shift assays to analyze pairing between SprC and czrB. (A) Specific complexes between radiolabeled SprC and czrB. (B) Gel retardation assay with SprC deletion mutant and radiolabeled czrB. (C) Gel retardation assay with czrB deletion mutant and radiolabeled SprC. (D) Gel retardation assays with radiolabeled SprC or SprC mutant and czrB or czrB compensatory mutant. The data were reproduced by three independent biological replicates.

This Article

  1. Published in Advance August 1, 2024, doi: 10.1261/rna.080122.124 RNA 30: 1451-1464

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