Influence of RBPs and enzymatic modification of RNA on SpRs. (A) The association between different RBPs and the SpR of the SS they recognize according to eCLIP (first four columns) and ATtRACT (last two columns). Color corresponds to the difference between median SpR of the RBP-bound SS and the sites that are not recognized by the protein. Asterisks correspond to Wilcoxon rank sum test FDR < 0.05. Hierarchical clusterization was performed with the average-linkage method (UPGMA) using cosine distances. (B) Violin and box plots demonstrating the SpRs of donor and acceptor SS in WT and SF3B4 knockdown cells. Color represents the cell line. P-values were calculated using a one-sided Wilcoxon signed-rank test. (C) Violin and box plots demonstrating the SpRs of the acceptor SS with very weak (0–1), weak (2–3), strong (4–5), or very strong (6–7) complementarity to U2 snRNA in WT and SF3B4 knockdown cells. Color represents the cell line. P-values were calculated using a one-sided Wilcoxon signed-rank test.
