Study of the RNA splicing kinetics via in vivo 5-EU labeling

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FIGURE 1.
FIGURE 1.

EU-RNA-seq experiment design. (A) Addition of 5-EU to the culture media followed by incubation for 10 min, 20 min, or 24 h. (B) EU labeled RNA purification using click reaction with biotin and streptavidin beads. (C) High-throughput sequencing of the cDNA library obtained from biotinylated RNA.

This Article

  1. Published in Advance July 24, 2024, doi: 10.1261/rna.079937.123 RNA 30: 1356-1373

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