Structural idiosyncrasies of glycyl T-box riboswitches among pathogenic bacteria

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FIGURE 7.
FIGURE 7.

EMSAs between increasing concentrations of (A) L. monocytogenes Stem I, (B) C. tetani Stem I, (C) S. pneumoniae Stem I, and (D) S. pyogenes Stem I domains from the respective glycyl T-boxes and 2 μM of tRNAGlyGCC. At the left, the Native PAGE images are shown, while at the right the binding curve of the bound tRNAGlyGCC against each Stem I is indicated. Also, the estimated Kd values for each complex are shown.

This Article

  1. RNA 30: 1328-1344