Kinetic and structural insights into the requirement of fungal tRNA ligase for a 2′-phosphate end

The His–Arg–Arg triad implicated in 2′-PO₄ binding is conserved in the tRNA ligases of fungal pathogens. The amino acid sequences in and flanking adenylyltransferase motifs III and V are aligned for the tRNA ligase enzymes of 18 human fungal pathogens designated as public health priorities by WHO. The defining glutamate of motif III and two lysines of motif V are shaded yellow. The histidine adjacent to motif III (corresponding to CthLIG His227) and the two arginine residues downstream from motif V (corresponding to CthLIG Arg334 and Arg337) are shaded cyan.