Identification of 16S LT helices across all phlya. (A,B) A phylogenetic tree of Proteobacteria, with leaf nodes colored based on shuffling-method z-scores (A; dark blue: no signal, light blue: weak signal, light red: intermediate signal, dark red: strong signal, see Fig. 1) or covariation-method results (B; dark blue: no evidence for stem with strong covariation, light blue: no evidence for stem with weak covariation, light red: evidence for stem with weak covariation, dark red: evidence for stem with strong covariation, see Fig. 2C,D). Gray leaf nodes signify no prediction. (C) The fraction of LT sequences from each phylum with an LT stem, predicted by either method (shuffling z-score ≥ 1 or covariation number of SSWs ≥ 15). Bacterial phyla, red labels; archaeal phyla, brown labels. Phyla with <25 LT sequences were grouped together in other phyla. The number of LTs for each phylum are noted in parentheses.
