Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis

Quantification of chromatin (local transcript)-bound subunits of THO. (A) Average in vivo binding profiles of Hpr1, Mft1, and Tho2 with (+Rho, green line) and without the Rho helicase (−Rho, blue line), across 5752 yeast protein-coding loci. The y-axis plots the log₂ ratio between IP samples (output) and input DNA. (B) Beeswarm plot summarizing the log₂ fold-enrichment of Tho2, Hpr1, Mft1, and Rrp6 ChIP-seq peaks. P-values: 0.05 > * > 0.01 > ** > 0.001 > ***, (NA) not applicable, (Nf) number of analyzed protein-coding genes, (Np) number of detected peaks. (C) Circos plots showing the genome-wide distribution of ChIP-seq peaks across the protein-coding loci in cells expressing (+Rho) or not expressing (−Rho) the Rho helicase.