Fluorescent labeling of RNA and DNA on the Hoogsteen edge using sulfinate chemistry

(A) Oceanobacillus iheyensis secondary structure map. (B) Denaturing PAGE showing before and after splicing time points (10 min splicing time) for a variety of O.i. samples labeled at different levels, with either DAAS by itself or with DAAS followed by AZ488-DBCO, along with unlabeled controls. Full-length (intron+ 5′-exon) and spliced bands are indicated to the right of the gel image. (C) The same gel as in B, before EtBr staining, imaged for AZ488 fluorescence.