FIGURE 4.
(A) Native PAGE showing the dye-labeled ssDNA1 and dye-labeled ssDNA2 samples loaded separately and as mixtures, alongside unlabeled controls. This gel was EtBr stained and imaged with an EtBr filter. (B) The same gel as in A, before EtBr staining, with the AZ488 and AZ680 fluorescence scans colored and merged. Purple coloration indicates both AZ488 and AZ680 fluorescence signal detected in the same portion of the gel. Note: All ssDNA1/ssDNA2 combination lanes were made from previously dye-labeled samples that were mixed immediately before gel loading—no labeling was done on ssDNA1/ssDNA2 mixtures.
