(A) Denaturing PAGE of the dye-labeled ssDNA1 and ssDNA2 samples, along with unlabeled controls. This gel was EtBr stained and imaged with an EtBr filter. (B) The same gel as in A, before EtBr staining, viewed using a laser/filter combination for Alexa Fluor 488 (used for imaging AZ488 fluorescence). (C) The same gel as in A, before EtBr staining, viewed using a laser/filter combination for Alexa Fluor 647 (used for imaging AZ680 fluorescence). (D) Picture of solutions containing the dye-labeled ssDNA1 and ssDNA2 samples in order of increasing DAAS labeling percentages. (E) Denaturing PAGE of mixtures of the dye-labeled ssDNA1 and ssDNA2 samples, along with unlabeled controls. This gel was EtBr stained and imaged with an EtBr filter. (F) The same gel as in E, before EtBr staining, imaged for AZ488 fluorescence. (G) The same gel as in E, before EtBr staining, imaged for AZ680 fluorescence. Note for (E–G): All ssDNA1/ssDNA2 combination lanes were made from previously dye-labeled samples that were mixed immediately before gel loading—no labeling was done on ssDNA1/ssDNA2 mixtures.
