Fluorescent labeling of RNA and DNA on the Hoogsteen edge using sulfinate chemistry

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FIGURE 2.
FIGURE 2.

(A) UV/Vis spectra of the AZ680-DBCO labeled 0 mM, 10 mM, 20 mM, and 40 mM DAAS ssDNA1 samples. (B) UV/Vis spectra of free AZ680-DBCO and AZ488-DBCO dyes. (C) Plot of average labeling percentage versus concentration of DAAS used to label the ssDNA1 substrate. Error bars indicate standard deviations for n = 3 independent replicates.

This Article

  1. Published in Advance June 5, 2023, doi: 10.1261/rna.079679.123 RNA 29: 1437-1451

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