Recoding UAG to selenocysteine in Saccharomyces cerevisiae

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FIGURE 4.
FIGURE 4.

Production of selenoprotein MsrB1 in S. cerevisiae. (A) Purified MsrB1 from S. cerevisiae shows conversion of l-Met-SO to l-Met which is comparable to an MsrB2 control. (B) Representative LC–MS/MS spectra show the presence of both Ser and Sec peptides of MsrB1. (C) Quantitative analysis of three separate spectra show ∼20% of MsrB1 contains Sec.

This Article

  1. Published in Advance June 6, 2023, doi: 10.1261/rna.079658.123 RNA 29: 1400-1410

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