Promoter-independent synthesis of chemically modified RNA by human DNA polymerase θ variants

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 7.
FIGURE 7.

Polθ steric-gate variant extension of RNA primers with 5′ modifications. (A) Schematic of RNA/DNA template. Black stars indicate the location of three consecutive 2′-ribose modifications at the 5′ terminus. (B–F) Denaturing gels showing time courses of PolθRP1 extension of the indicated 5′ modified RNA/DNA template in the presence NTPs and MgCl2.

This Article

  1. Published in Advance April 27, 2023, doi: 10.1261/rna.079396.122 RNA 29: 1288-1300

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA