Promoter-independent synthesis of chemically modified RNA by human DNA polymerase θ variants

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 6.
FIGURE 6.

Polθ steric-gate variant synthesis of phosphorothioate-modified RNA. Denaturing gel showing a time course of PolθRP1 DNA-dependent RNA synthesis in the presence of all four 1′-phosphorothioate-NTP analogs with 1 mM DTT, 5 mM MgCl2, and 1 mM MnCl2.

This Article

  1. Published in Advance April 27, 2023, doi: 10.1261/rna.079396.122 RNA 29: 1288-1300

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA