Promoter-independent synthesis of chemically modified RNA by human DNA polymerase θ variants

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FIGURE 5.
FIGURE 5.

Polθ steric-gate variant incorporation of 2′-O-modified ribonucleotides. (A) Schematic of RNA/DNA primer–templates. Red text, RNA; black text, DNA. (B,C) Denaturing gels showing time courses of PolθRP1 extension of the indicated RNA/DNA templates in the presence of the indicated 2′-O-Me-NTPs and divalent cation.

This Article

  1. Published in Advance April 27, 2023, doi: 10.1261/rna.079396.122 RNA 29: 1288-1300

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