FIGURE 3.
Comparison of Polθ steric-gate variant to T7 RNAP. (A,B) Denaturing gels showing time courses of PolθRP1 and T7 RNAP on the indicated RNA/DNA primer–templates in the presence of canonical NTPs. T7 RNAP and PolθRP1 were preincubated with the RNA/DNA template for 10 min in panel B where indicated. % RNA extension indicated (B). (C,D) Denaturing gels showing RNA extension by PolθRP1 and T7 RNAP following a 10 min preincubation period on the indicated RNA/DNA with the indicated NTPs.
