Promoter-independent synthesis of chemically modified RNA by human DNA polymerase θ variants

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FIGURE 1.
FIGURE 1.

Structure–function analysis of Taq steric-gate variant. (A) Superposition of Taq DNAP and Polθ crystal structures. Superposition of Taq DNAP (blue; PDB ID: 1qss) and Polθ (green; PDB ID: 4x0q) bound to DNA/DNA templates with incoming ddGTP shows the close alignment of their respective steric-gate residues. (B) Denaturing gel showing premature termination of Taq DNAP E615G on the indicated DNA/DNA primer–template in the presence of NTPs (left). Denaturing gel showing exonuclease activity by Taq DNAP E615G on the indicated RNA/DNA primer–template in the presence of NTPs (right).

This Article

  1. Published in Advance April 27, 2023, doi: 10.1261/rna.079396.122 RNA 29: 1288-1300

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