Translation regulation by a guanidine-II riboswitch is highly tunable in sensitivity, dynamic range, and apparent cooperativity

(A) Schematic of the high-throughput mutational analysis of RNA translation initiation. (B) Distribution of functional K_1/2 values as ΔΔG_Sensitivity (n = 7222). (C) Distribution of functional amplitude values (n = 7222). (D) Distribution of single mutant Hill coefficient values (n = 127). (E) Distribution of function parameter values (n = 7222). Wild-type value for all parameters is indicated on the y-axis. (F) Plot of K_1/2 versus amplitude for all functional variants (dark blue), all functional single mutants (yellow), and the wild type (red). Dotted lines indicate the intersection of wild-type values.