The impact of iron availability on the TfR1 and CDC14A mRNA stability. Cells were treated with either 100 µg/mL FAC or 100 µM DFO following the pulse labeling with the EU as described in Materials and Methods. (A) Impact of the DFO and FAC treatments on the TfR1 mRNA stability. (B) Impact of the DFO and FAC treatments on the stability of the CDC14A plus-IRE variant. The difference between the stability of the variant under iron-poor and iron-rich conditions reaches statistical significance by 10 h of treatment. (C) Impact of the DFO and FAC treatments on the stability of the CDC14A non-IRE variant. (D) Relative abundance of the CDC14A and TfR1 mRNAs immediately following the EU pulse labeling. Statistical significance was analyzed by two-tailed Student's t-test. (**) P = 0.003. All error bars represent ±SEM of three biological replicates except the 5 h time point for the CDC14A non-IRE variant which could only be detected within two sets of the biological replicates. The t1/2 values calculated from the first-order rate constants obtained from the slopes are summarized in Table 1.
