Artifacts and biases of the reverse transcription reaction in RNA sequencing

Overview of reverse transcription components and characteristics at the heart of intrasample quantification biases. Graphical overview of the intrasample biases akin to the reverse transcription reaction. The blue ellipses of “priming method” and “reverse transcriptase” overlap with the “RNA” circle as it is from the interaction of these factors that the biases originate. This figure was created using BioRender.