Model for LLPS of Y14. (A) Ribbon diagram showing the RRM of Y14, in which seven exposed aromatic side chains are presented. (B) Diagram shows net charge of each charged region of Y14. Note that 16 negatively charged residues and seven positively charged residues present in the RRM give it a net charge of approximately −9 at pH 7. Plot graph shows total net charge of the IDRs of indicated Y14 versions against their droplet size (the droplet size of ΔN1N3 was measured at pH 7.5 and all others at pH 6.5). (C) Y14 in concert with lncRNAs (such as HOTAIRM1) undergoes LLPS during DNA damage (lightning bolt) to facilitate repair. Colored ovals except for green (Y14) represent Y14- or lncRNA-associated DNA repair factors.
